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avidin biotin complex abc peroxidase reagent  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin biotin complex abc peroxidase reagent
    Avidin Biotin Complex Abc Peroxidase Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+peroxidase+complex+abc+reagent/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13061616-236-13-23
    Average 99 stars, based on 12758 article reviews
    avidin biotin complex abc peroxidase reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: The sodium-glucose cotransporter-2 inhibitor canagliflozin alleviates endothelial dysfunction in a rat bypass model.
    Article Snippet: Ischemia/reperfusion (IR) injury during coronary artery bypass grafting surgery has detrimental impacts on endothelial integrity and function.. Studies have suggested that canagliflozin (CANA) mitigates the risk of cardiovascular events, independently of diabetes.. We hypothesized that the supplementation and/or administration of CANA protects vascular grafts in a bypass model in non-diabetic Lewis rats.

    Article Title: Fiber Tracts Anomalies in APPxPS1 Transgenic Mice Modeling Alzheimer's Disease
    Article Snippet: Sections were then incubated overnight at RT with a C-terminal antineurofilament M (145KD) primary antibody (AB1987; Chemicon, Temecula, Calif) diluted 1 : 1000 in serum. .. The following day sections were incubated in a secondary biotinylated antirabbit antibody solution (Sigma, St. Louis, Mo; 1 : 200, 1.5 hours at RT) and then in an avidin-biotin peroxidase complex (ABC) reagent (Vectastain Elite ABC-Kit, Vector Laboratories, USA, 1 : 800, 1.5 hours at RT). ..

    Article Title: Topographical distribution of corticotropin-releasing factor type 2 (CRF 2 ) receptor-like immunoreactivity in the rat dorsal raphe nucleus: co-localization with tryptophan hydroxylase
    Article Snippet: Sections were washed in 0.01 M PBS containing 0.04% normal rabbit serum 2 times for 15 min, and incubated for 2 h at RT in biotin-conjugated donkey anti-goat secondary antibody (1:200; Cat. No.705-066-147, Jackson ImmunoResearch) in 0.01 M PBS containing 0.04% normal rabbit serum and 0.03% Triton X-100 to visualize CRF 2 receptor-like immunoreactivity. .. After incubation with secondary antibody, sections were washed in 0.01 M PBS containing 0.04% normal rabbit serum 2 times for 15 min, and then placed in an avidin-biotin-peroxidase complex (ABC) reagent (Cat. No. PK-6106; Vector Laboratories, Burlingame, CA, USA) at 1:200 in 0.01 M PBS for 90 min. ..

    Article Title: Neuroanatomical and Immunohistological Study of the Main and Accessory Olfactory Bulbs of the Meerkat ( Suricata suricatta )
    Article Snippet: .. Biotinylated conjugates of LEA and BSI-B 4 were used for this study (see details in ). (i) We used 3% hydrogen peroxide (H 2 O 2 ) solution to suppress the endogenous peroxidase activity. (ii) After two washes in 0.1 M phosphate-buffered (pH 7.2) solution (PB), (iii) the sections were incubated for 30 min in a 2% bovine serum albumin (BSA) in PB. (iv) Slides were then incubated overnight at 4 °C with LEA and BSI-B 4 lectins, independently, in a 0.5% BSA solution. (v) After 2 washes in PB, (vi) they were incubated during 1.5 h at room temperature with avidin-biotin-peroxidase complex (ABC) reagent (Vectastain; Vector Laboratories, Burlingame, CA, USA). (vi) The slides were developed in the same way as the slides in the IHQ protocol. ..

    Article Title: Dual‐targeting therapy against HER3 / MET in human colorectal cancers
    Article Snippet: After washing, they were incubated with Block Ace (DS Pharma Biomedical Co., Ltd.) at room temperature for 2 h. They were then incubated with Ab4 anti‐HER3 (10 μg/mL) or Ab57 anti‐MET mAb (10 μg/mL) primary rat mAb diluted in 1% BSA‐PBS at 4°C overnight. .. After washing, sections were incubated with biotinylated goat anti‐rat IgG (Vector Laboratories) diluted 1:200 in 2% BSA‐PBS at room temperature for 30 min. After washing, samples were treated with avidin‐biotin‐peroxidase complex (ABC) reagent (Vector Laboratories) diluted 1:50 in 2% BSA‐PBS at room temperature for 30 min. After washing, sections were incubated with 0.05% 3,3′‐diaminobenzidine (Dojin Chemicals) and 0.01% H 2 O 2 in 0.1 M Tris–HCl (pH 7.4), and then counterstained with hematoxylin. .. Sections were dehydrated, cleared in xylene, and mounted in Permount (Fisher Scientific).

    Article Title: Ischemia/reperfusion protection compositions and methods of using
    Article Snippet: .. Sections were then incubated 30 min with biotinylated goat anti-mouse IgG (5 μg/ml in blocking solution) and 30 min with avidin-biotin-peroxidase complex (ABC) reagent (both reagents from Vector Laboratories, Burlingame, Calif.). .. Color development was from 1 to 6 min in 0.6 g/ml 3,3′-diaminobenzidine (Sigma).

    Incubation:

    Article Title: The sodium-glucose cotransporter-2 inhibitor canagliflozin alleviates endothelial dysfunction in a rat bypass model.
    Article Snippet: Ischemia/reperfusion (IR) injury during coronary artery bypass grafting surgery has detrimental impacts on endothelial integrity and function.. Studies have suggested that canagliflozin (CANA) mitigates the risk of cardiovascular events, independently of diabetes.. We hypothesized that the supplementation and/or administration of CANA protects vascular grafts in a bypass model in non-diabetic Lewis rats.

    Article Title: Fiber Tracts Anomalies in APPxPS1 Transgenic Mice Modeling Alzheimer's Disease
    Article Snippet: Sections were then incubated overnight at RT with a C-terminal antineurofilament M (145KD) primary antibody (AB1987; Chemicon, Temecula, Calif) diluted 1 : 1000 in serum. .. The following day sections were incubated in a secondary biotinylated antirabbit antibody solution (Sigma, St. Louis, Mo; 1 : 200, 1.5 hours at RT) and then in an avidin-biotin peroxidase complex (ABC) reagent (Vectastain Elite ABC-Kit, Vector Laboratories, USA, 1 : 800, 1.5 hours at RT). ..

    Article Title: Topographical distribution of corticotropin-releasing factor type 2 (CRF 2 ) receptor-like immunoreactivity in the rat dorsal raphe nucleus: co-localization with tryptophan hydroxylase
    Article Snippet: Sections were washed in 0.01 M PBS containing 0.04% normal rabbit serum 2 times for 15 min, and incubated for 2 h at RT in biotin-conjugated donkey anti-goat secondary antibody (1:200; Cat. No.705-066-147, Jackson ImmunoResearch) in 0.01 M PBS containing 0.04% normal rabbit serum and 0.03% Triton X-100 to visualize CRF 2 receptor-like immunoreactivity. .. After incubation with secondary antibody, sections were washed in 0.01 M PBS containing 0.04% normal rabbit serum 2 times for 15 min, and then placed in an avidin-biotin-peroxidase complex (ABC) reagent (Cat. No. PK-6106; Vector Laboratories, Burlingame, CA, USA) at 1:200 in 0.01 M PBS for 90 min. ..

    Article Title: Neuroanatomical and Immunohistological Study of the Main and Accessory Olfactory Bulbs of the Meerkat ( Suricata suricatta )
    Article Snippet: .. Biotinylated conjugates of LEA and BSI-B 4 were used for this study (see details in ). (i) We used 3% hydrogen peroxide (H 2 O 2 ) solution to suppress the endogenous peroxidase activity. (ii) After two washes in 0.1 M phosphate-buffered (pH 7.2) solution (PB), (iii) the sections were incubated for 30 min in a 2% bovine serum albumin (BSA) in PB. (iv) Slides were then incubated overnight at 4 °C with LEA and BSI-B 4 lectins, independently, in a 0.5% BSA solution. (v) After 2 washes in PB, (vi) they were incubated during 1.5 h at room temperature with avidin-biotin-peroxidase complex (ABC) reagent (Vectastain; Vector Laboratories, Burlingame, CA, USA). (vi) The slides were developed in the same way as the slides in the IHQ protocol. ..

    Article Title: Dual‐targeting therapy against HER3 / MET in human colorectal cancers
    Article Snippet: After washing, they were incubated with Block Ace (DS Pharma Biomedical Co., Ltd.) at room temperature for 2 h. They were then incubated with Ab4 anti‐HER3 (10 μg/mL) or Ab57 anti‐MET mAb (10 μg/mL) primary rat mAb diluted in 1% BSA‐PBS at 4°C overnight. .. After washing, sections were incubated with biotinylated goat anti‐rat IgG (Vector Laboratories) diluted 1:200 in 2% BSA‐PBS at room temperature for 30 min. After washing, samples were treated with avidin‐biotin‐peroxidase complex (ABC) reagent (Vector Laboratories) diluted 1:50 in 2% BSA‐PBS at room temperature for 30 min. After washing, sections were incubated with 0.05% 3,3′‐diaminobenzidine (Dojin Chemicals) and 0.01% H 2 O 2 in 0.1 M Tris–HCl (pH 7.4), and then counterstained with hematoxylin. .. Sections were dehydrated, cleared in xylene, and mounted in Permount (Fisher Scientific).

    Article Title: Ischemia/reperfusion protection compositions and methods of using
    Article Snippet: .. Sections were then incubated 30 min with biotinylated goat anti-mouse IgG (5 μg/ml in blocking solution) and 30 min with avidin-biotin-peroxidase complex (ABC) reagent (both reagents from Vector Laboratories, Burlingame, Calif.). .. Color development was from 1 to 6 min in 0.6 g/ml 3,3′-diaminobenzidine (Sigma).

    Activity Assay:

    Article Title: Neuroanatomical and Immunohistological Study of the Main and Accessory Olfactory Bulbs of the Meerkat ( Suricata suricatta )
    Article Snippet: .. Biotinylated conjugates of LEA and BSI-B 4 were used for this study (see details in ). (i) We used 3% hydrogen peroxide (H 2 O 2 ) solution to suppress the endogenous peroxidase activity. (ii) After two washes in 0.1 M phosphate-buffered (pH 7.2) solution (PB), (iii) the sections were incubated for 30 min in a 2% bovine serum albumin (BSA) in PB. (iv) Slides were then incubated overnight at 4 °C with LEA and BSI-B 4 lectins, independently, in a 0.5% BSA solution. (v) After 2 washes in PB, (vi) they were incubated during 1.5 h at room temperature with avidin-biotin-peroxidase complex (ABC) reagent (Vectastain; Vector Laboratories, Burlingame, CA, USA). (vi) The slides were developed in the same way as the slides in the IHQ protocol. ..

    Blocking Assay:

    Article Title: Ischemia/reperfusion protection compositions and methods of using
    Article Snippet: .. Sections were then incubated 30 min with biotinylated goat anti-mouse IgG (5 μg/ml in blocking solution) and 30 min with avidin-biotin-peroxidase complex (ABC) reagent (both reagents from Vector Laboratories, Burlingame, Calif.). .. Color development was from 1 to 6 min in 0.6 g/ml 3,3′-diaminobenzidine (Sigma).



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